biotinylated goat anti il 13 ab  (R&D Systems)

Journal:

Article Title: IL-13 receptor ? 2 down-modulates granulomatous inflammation and prolongs host survival in schistosomiasis

doi: 10.1073/pnas.0305064101

Figure Lengend Snippet: IL-13Rα2 down-modulates granulomatous inflammation and prolongs survival in chronic S. mansoni infection. (a) C57BL/6 mice were infected percutaneously with 25–30 cercariae of S. mansoni. Uninfected (“wk 0,” n = 5) and infected mice (n = 5 weekly) were bled from wk 4 to wk 18 postinfection (open squares) to measure serum IL-13Rα2 levels; mean granuloma volumes were also determined (n = 5) from wk 7 to wk 20 (filled circles). The dashed line represents the mean level of serum IL-13Rα2 in naive mice. (b) BALB/c male (filled squares, n = 20), BALB/c female (filled circles, n = 20), IL-13Rα2–/– male (open squares, n = 18), and IL-13Rα2–/– female (open circles, n = 15) mice were infected percutaneously with 25–30 cercariae of S. mansoni. The median survival time for all IL-13Rα2–/– mice after infection was 15 wk (*, P = 0.0002). Survival was analyzed and compared with WT mice by log-rank test using prism 3.0 (GraphPad, San Diego). (c) BALB/c (wk 8, n = 36; wk 12, n = 36) and IL-13Rα2–/– (wk 8, n = 20; wk 12, n = 28) mice were infected and killed on wk 8 and wk 12 postinfection. The circles represent average granuloma measurements for individual mice from three independent experiments. Approximately 30 granulomas were measured in each animal. Relevant statistical comparisons are shown. (d) Uninfected and infected BALB/c(n = 5 per time point, filled squares) and IL-13Rα2–/– (open circles) mice were bled to quantify serum IL-13Rα2 levels at the times indicated. (e) Mice were infected, and liver granuloma size was measured at wk 12 postinfection in the surviving animals: BALB/c mice (n = 9), IL-13Rα2–/– mice treated with control IgG (n = 7), and IL-13Rα2–/– mice treated from wk 6 through wk 12 with 200 μg of soluble IL-13Rα2-Fc (sIL-13Rα2-Fc) (n = 9) injected three times weekly. BALB/c vs. sIL-13Rα2-Fc treated IL-13Rα2–/–, P = 0.011. Other relevant statistical comparisons are shown.

Article Snippet: The detection Ab biotinylated goat anti-mouse IL-13Rα2 (R & D Systems) was added for 2 h at 37°C.

Techniques: Infection, Injection

Journal:

Article Title: IL-13 receptor ? 2 down-modulates granulomatous inflammation and prolongs host survival in schistosomiasis

doi: 10.1073/pnas.0305064101

Figure Lengend Snippet: Chronically infected IL-13Rα2–/– mice lack viable parasite eggs and newly formed granulomas in the liver and develop significant portal obstruction. BALB/c and IL-13Rα2–/– mice were infected with S. mansoni, and survivors were killed at wk 38 (n ≥ 10 analyzed per group). (a) Liver granuloma size was measured in infected BALB/c mice but could not be determined (ND) in IL-13Rα2–/– mice due to the absence of newly deposited mature eggs. (b) Representative hepatic granuloma from chronically infected BALB/c mice (Giemsa stain). (c) Chronically infected IL-13Rα2–/– mice lack viable parasite eggs in the liver and are devoid of active granulomatous lesions (Giemsa stain). (d) Fibrosis (μmol of hydroxyproline per 1.0 × 104 eggs) is significantly exacerbated in IL-13Rα2–/– mice after 38 wk of infection (*, P < 0.001). (e) Liver sections were prepared from chronically infected (38 wk) BALB/c mice and stained with the collagen-specific stain picrosirius red. Collagen deposition was localized within the granulomas. (f) Liver sections were prepared from chronically infected (38 wk) IL-13Rα2–/– mice and stained with picrosirius red. Large densely staining bands of collagen were seen throughout the liver. (g) Tissues were digested to enumerate egg burdens. The total tissue egg burden per worm pair, total eggs per gut (small and large intestine), and total eggs per liver and lung are shown as averages ± SE.

Article Snippet: The detection Ab biotinylated goat anti-mouse IL-13Rα2 (R & D Systems) was added for 2 h at 37°C.

Techniques: Infection, Giemsa Stain, Staining

Journal:

Article Title: IL-13 receptor ? 2 down-modulates granulomatous inflammation and prolongs host survival in schistosomiasis

doi: 10.1073/pnas.0305064101

Figure Lengend Snippet: Serum IL-13Rα2 increases in subjects chronically infected with S. mansoni. Schistosomiasis mansoni subjects (380) living in an endemic region in Uganda with evidence of chronic infection (eggs detectable in stool) were bled and then treated with praziquantel. One hundred uninfected controls from North America were also bled to establish a normal range for the soluble IL-13Rα2. Receptor levels in serum are shown in box and whisker plots. The median response is represented by the horizontal black line within a box that represents the 25th to 75th percentile range. Whiskers represent the range of data excluding outliers. The means and P values for each group are also shown (each patient group compared with uninfected controls). Patients in the low 0–200 eggs per g group were also significantly different from the heavily infected (>1,000 eggs per g of feces) group (P = 0.03).

Article Snippet: The detection Ab biotinylated goat anti-mouse IL-13Rα2 (R & D Systems) was added for 2 h at 37°C.

Techniques: Infection, Whisker Assay